Review



pgadt7 vector  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    TaKaRa pgadt7 vector
    Pgadt7 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3980 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgadt7+ad+vector/pGADT7+AD+Vector/pmc13188844-81-52-54
    Average 99 stars, based on 3980 article reviews
    pgadt7 vector - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: pseudo-GhFAD2-1 Is a lncRNA Involved in Regulating Cottonseed Oleic and Linoleic Acid Ratios and Seed Size in Gossypium hirsutum.
    Article Snippet: .. YZ- 1 and then was introduced into the pGADT7 AD vector (Clontech). ..

    Article Title: Reenacting a mouse genetic evolutionary arms race in yeast reveals that SLXL1/SLX compete with SLY1/2 for binding to Spindlins.
    Article Snippet: .. Prey proteins were expressed from a modified pGADT7 AD vector (pGAD; Takara), which expresses a protein of interest fused to a GAL4 AD (amino acids 768 to 881). ..

    Article Title: Ethylene enhances peanut seed germination by modulating hormonal and metabolic pathways
    Article Snippet: Ethylene influences various physiological processes especially seed germination in plants.. Through this study of ethylene on peanut seed germination, we found that soaking seeds in a 0.2 mM ethylene solution for 12 h, followed by water soaking, optimally promotes germination.. To understand the mechanisms involved, we measured abscisic acid (ABA), gibberellins (GA), crude fat, and 18 fatty acids using GC-MS and also analyzed gene expression related to hormone pathways, fatty acid degradation, and cell wall loosening in both control and ethylene-treated seeds.

    Article Title: pseudo‐GhFAD2‐1 Is a lncRNA Involved in Regulating Cottonseed Oleic and Linoleic Acid Ratios and Seed Size in Gossypium hirsutum
    Article Snippet: .. YZ‐1 and then was introduced into the pGADT7 AD vector (Clontech). ..

    Article Title: Brassinolides Enhance Seed Kernel Development via CoSMT2 -Mediated 24-Ethylsterol Accumulation in Camellia oleifera .
    Article Snippet: Brassinolide (BL), the sixth major plant hormone, is synthesized from phytosterols.. At present, research on the relationship among BL, phytosterols, and C. oleifera fruit development remains limited.. In this study, we found that 0.5 mg/L of BL promoted the growth of seed kernel cells and increased the growth of ‘Huashuo’ seeds.

    Article Title: Dual regulation of appressorium development in the maize pathogen Bipolaris maydis : BmOPY2-mediated hydrophobic surface recognition and pectin recognition
    Article Snippet: .. The PCR amplicons containing BmSTE50 and BmOPY2 coding sequences were cloned into the pGBKT7 DNA-BD vector (Clontech) and pGADT7 AD vector (Clontech), respectively, both pre-digested with BamHI and EcoRI. .. The resulting plasmids (pBaitBmSte50 and pPreyBmOpy2) were transformed into S. cerevisiae strains Y2HGold and Y187, respectively, using the lithium acetate transformation method ( ).

    Article Title: Red Light-Activated Reversible Inhibition of Protein Functions by Assembled Trap
    Article Snippet: .. The fragments of Dr BphP were cloned into the pGBKT7 DNA-BD vector (Takara, Cat.#630443), while LDB-3 and LDB-14 fragments connected by 1× linker, 2× linkers, 3× linkers, or 4× linkers were cloned into the pGADT7 AD vector (Takara, Cat.#630442). ..

    Modification:

    Article Title: Reenacting a mouse genetic evolutionary arms race in yeast reveals that SLXL1/SLX compete with SLY1/2 for binding to Spindlins.
    Article Snippet: .. Prey proteins were expressed from a modified pGADT7 AD vector (pGAD; Takara), which expresses a protein of interest fused to a GAL4 AD (amino acids 768 to 881). ..

    Y1H Assay:

    Article Title: Ethylene enhances peanut seed germination by modulating hormonal and metabolic pathways
    Article Snippet: Ethylene influences various physiological processes especially seed germination in plants.. Through this study of ethylene on peanut seed germination, we found that soaking seeds in a 0.2 mM ethylene solution for 12 h, followed by water soaking, optimally promotes germination.. To understand the mechanisms involved, we measured abscisic acid (ABA), gibberellins (GA), crude fat, and 18 fatty acids using GC-MS and also analyzed gene expression related to hormone pathways, fatty acid degradation, and cell wall loosening in both control and ethylene-treated seeds.

    Library Screening:

    Article Title: Ethylene enhances peanut seed germination by modulating hormonal and metabolic pathways
    Article Snippet: Ethylene influences various physiological processes especially seed germination in plants.. Through this study of ethylene on peanut seed germination, we found that soaking seeds in a 0.2 mM ethylene solution for 12 h, followed by water soaking, optimally promotes germination.. To understand the mechanisms involved, we measured abscisic acid (ABA), gibberellins (GA), crude fat, and 18 fatty acids using GC-MS and also analyzed gene expression related to hormone pathways, fatty acid degradation, and cell wall loosening in both control and ethylene-treated seeds.

    Clone Assay:

    Article Title: Ethylene enhances peanut seed germination by modulating hormonal and metabolic pathways
    Article Snippet: Ethylene influences various physiological processes especially seed germination in plants.. Through this study of ethylene on peanut seed germination, we found that soaking seeds in a 0.2 mM ethylene solution for 12 h, followed by water soaking, optimally promotes germination.. To understand the mechanisms involved, we measured abscisic acid (ABA), gibberellins (GA), crude fat, and 18 fatty acids using GC-MS and also analyzed gene expression related to hormone pathways, fatty acid degradation, and cell wall loosening in both control and ethylene-treated seeds.

    Article Title: Dual regulation of appressorium development in the maize pathogen Bipolaris maydis : BmOPY2-mediated hydrophobic surface recognition and pectin recognition
    Article Snippet: .. The PCR amplicons containing BmSTE50 and BmOPY2 coding sequences were cloned into the pGBKT7 DNA-BD vector (Clontech) and pGADT7 AD vector (Clontech), respectively, both pre-digested with BamHI and EcoRI. .. The resulting plasmids (pBaitBmSte50 and pPreyBmOpy2) were transformed into S. cerevisiae strains Y2HGold and Y187, respectively, using the lithium acetate transformation method ( ).

    Article Title: Red Light-Activated Reversible Inhibition of Protein Functions by Assembled Trap
    Article Snippet: .. The fragments of Dr BphP were cloned into the pGBKT7 DNA-BD vector (Takara, Cat.#630443), while LDB-3 and LDB-14 fragments connected by 1× linker, 2× linkers, 3× linkers, or 4× linkers were cloned into the pGADT7 AD vector (Takara, Cat.#630442). ..

    Polymerase Chain Reaction:

    Article Title: Dual regulation of appressorium development in the maize pathogen Bipolaris maydis : BmOPY2-mediated hydrophobic surface recognition and pectin recognition
    Article Snippet: .. The PCR amplicons containing BmSTE50 and BmOPY2 coding sequences were cloned into the pGBKT7 DNA-BD vector (Clontech) and pGADT7 AD vector (Clontech), respectively, both pre-digested with BamHI and EcoRI. .. The resulting plasmids (pBaitBmSte50 and pPreyBmOpy2) were transformed into S. cerevisiae strains Y2HGold and Y187, respectively, using the lithium acetate transformation method ( ).



    Similar Products

    99
    TaKaRa pgadt7 vector
    Pgadt7 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgadt7+ad+vector/pGADT7+AD+Vector/pmc13188844-81-52-54
    Average 99 stars, based on 1 article reviews
    pgadt7 vector - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    TaKaRa empty pgadt7 vector
    Empty Pgadt7 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgadt7+ad+vector/pGADT7+AD+Vector/pmc13161086-261-5-36
    Average 99 stars, based on 1 article reviews
    empty pgadt7 vector - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    TaKaRa pgadt7 prey vector
    Pgadt7 Prey Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgadt7+ad+vector/pGADT7+AD+Vector/pm41872729-391-11-14
    Average 99 stars, based on 1 article reviews
    pgadt7 prey vector - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    Image Search Results